| one publication added to basket [3022] | Dietary ascorbic acid requirements during the hatchery production of turbot larvae
Merchie, G.; Lavens, P.; Dhert, Ph.; Garcia Ulloa Gómez, M.; Nelis, H.; De Leenheer, A.P.; Sorgeloos, P. (1996). Dietary ascorbic acid requirements during the hatchery production of turbot larvae. J. Fish Biol. 49(4): 573-583. http://dx.doi.org/10.1111/j.1095-8649.1996.tb00055.x In: Journal of Fish Biology. Fisheries Society of the British Isles: London,New York,. ISSN 0022-1112; e-ISSN 1095-8649, more |  |
| Authors | | Top | - Merchie, G.
- Lavens, P., more
- Dhert, Ph., more
- Garcia Ulloa Gómez, M.
| - Nelis, H.
- De Leenheer, A.P.
- Sorgeloos, P., more
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| Abstract | The effect of the high ascorbic acid (AA) levels transferred through enriched live food was evaluated for turbot Scophthalmus maximus larvae in two consecutive feeding experiments. The same feeding strategy was applied to all treatments, except for the AA content in the live food which was manipulated through bioencapsulation with ascorbyl palmitate. This resulted finally in a low, medium and high-AA treatment. The AA incorporation levels in the turbot larvae (up to 1400 µg AA g DW-1) were correlated with the AA content of the live food administered. However, feeding the high AA concentration resulted in the same values as for the medium treatment, indicating a saturation of the body AA reserves. Under standard culture conditions, no differences in growth nor overall survival could be detected among the different groups, illustrating that the dietary AA requirements of larval turbot are met by non-enriched live food containing already 500 µg AA g DW-1. The larvae of the high-AA treatment, however, showed a better pigmentation rate (47 and 32% for experiments 1 and 2, respectively) compared to the other groups (35 and 25%, respectively). Evaluation of the physiological condition applying in a salinity stress test revealed an improvement by feeding extra AA, significantly in the medium-AA treatment. Though not significantly different, cumulative mortalities after challenge with Vibrio anguillarum amounted to 50% for the control v. 40% for the fish fed medium and high-AA diets, respectively. Moreover, the onset of mortalities in this study was slower (not significantly) for the fish fed the extra AA. |
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