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Distinct dynamics of Vibrio parahaemolyticus populations in two farming models
Yang, Q.; Wang, Q.; Wu, J.; Zhang, Y.; Wei, D.; Qu, B.; Liu, Y.; Fu, S. (2022). Distinct dynamics of Vibrio parahaemolyticus populations in two farming models. J. Appl. Microbiol. 133(3): 1146-1155. https://dx.doi.org/10.1111/jam.15217
In: Journal of Applied Microbiology. Blackwell Science: Oxford. ISSN 1364-5072; e-ISSN 1365-2672, more
Peer reviewed article  
Available in  Authors 
Keyword
    Vibrio parahaemolyticus (Fujino, Okuno, Nakada, Aoyama, Fukai, Mukai & Ueho, 1951) Sakazaki, Iwanami & Fukumi, 1963 [WoRMS]
Author keywords
    MLST; re-circulating aquaculture system; traditional farming; Vibrio parahaemolyticus; whole-genome sequencing
Authors  Top 
  • Yang, Q., more
  • Wang, Q.
  • Wu, J.
  • Zhang, Y.
  • Wei, D.
  • Qu, B.
  • Liu, Y.
  • Fu, S.
Abstract
    Aims

    Despite the recent prosperity of shrimp cultivation in China, very little is known about how different shrimp farming models influence the dynamics of Vibrio parahaemolyticus populations and the antibiotic resistance of this bacterium.

    Methods and Results

    To this end, we conducted continuous surveillance of V. parahaemolyticus on four farms over 3 years: two traditional shrimp farms with daily water exchange and two farms operated in the recirculating aquaculture systems (RAS). No antibiotics were used in these farms to exclude the potential impacts of antibiotics on the emergence of antibacterial resistance. Multilocus sequence typing was utilized to characterize the dynamics of V. parahaemolyticus populations. Whole-genome sequencing (WGS) was conducted to determine the representative sequence types (STs) at each farm. Results revealed that the population structure of V. parahaemolyticus remained stable over time in both RAS farms, with only nine and four STs observed at each. In contrast, annual replacement of V. parahaemolyticus populations was observed in traditional farms with 26 and 28 STs identified in rearing water. WGS of 50 isolates divided them into five clusters, of which ST917a isolates harboured a genomic island that disrupted the gene recA. Pair-wised genomic comparison of isolates from the same STs showed that they were genetically related but belonged to different clones associated with geographical distribution.

    Conclusions

    These results suggested that RAS presented a specific ecological niche by minimizing the water exchanges with the external environment. In contrast, traditional farming might pose a food safety issue by introducing new V. parahaemolyticus populations with antibiotic resistance genes.

    Significance and Impact of the Study

    Our results expose the potential food safety issue associated with conventional agriculture and should encourage the development of preventive strategies to reduce the emergence of resistant V. parahaemolyticus populations.

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