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Metabolism, reproduction and moulting as endpoints to study endocrine disruption of Neomysis integerin the Scheldt Estuary [Metabolisme, reproductie en vervelling als eindpunten voor het bestuderen van endocriene verstoring bij Neomysis integer in het Schelde-estuarium] Citation Ghekiere A., Verslycke T., De Smet L., Van Beeumen J., Janssen C.R.; Environmental toxicology research group - Ugent; (2004): Metabolism, reproduction and moulting as endpoints to study endocrine disruption of Neomysis integerin the Scheldt Estuary. https://marineinfo.org/id/dataset/56 Contact: Ghekiere, An Availability:  This dataset is licensed under a Creative Commons Attribution 4.0 International License.Description This dataset results out of the study into ecotoxicological evaluation of the effects of endocrine disrupting substances occurring in the Scheldt estuary on resident mysid shrimp populations (laboratory and field studies). more The data results from a number of lab tests.To evaluate the possible effects of the pollutants on mysid shrimp, acute and chronic test will be performed in the laboratory. Different endpoints to study endocrine disruption are metabolism, reproduction, vitellogenesis and moulting. Scope Themes: Biology, Biology > Invertebrates, Environmental quality/pollution Keywords: Biomarkers, Crustacea, Energy, Estrogens, Metabolism, Moulting, Reproduction, Vitellogenesis, ANE, Scheldt Estuary, EurOBIS calculated BBOX, ANE, Netherlands, Westerschelde, Belgium, Zeeschelde, ANE, Netherlands, Westerschelde, Bath, ANE, Netherlands, Westerschelde, Hansweert, ANE, Netherlands, Westerschelde, Terneuzen, ANE, Netherlands, Westerschelde, Verdronken Land van Saeftinge, ANE, Netherlands, Westerschelde, Vlissingen, Belgium, Zeeschelde, Antwerp, Belgium, Zeeschelde, Doel, Neomysis integer (Leach, 1814) Geographical coverage ANE, Scheldt Estuary [Marine Regions] EurOBIS calculated BBOX Stations Bounding Box Coordinates: MinLong: 4,078; MinLat: 51,365 - MaxLong: 4,225; MaxLat: 51,37 [WGS84] ANE, Netherlands, Westerschelde [Marine Regions] Belgium, Zeeschelde [Marine Regions] ANE, Netherlands, Westerschelde, Bath Stations [Marine Regions] S12 Coordinates: Long: 4,225; Lat: 51,365 [WGS84] ANE, Netherlands, Westerschelde, Hansweert Stations [Marine Regions] S07 Coordinates: Long: 4; Lat: 51,4367 [WGS84] ANE, Netherlands, Westerschelde, Terneuzen Stations [Marine Regions] S04 Coordinates: Long: 3,825; Lat: 51,345 [WGS84] ANE, Netherlands, Westerschelde, Verdronken Land van Saeftinge Stations [Marine Regions] S09 Coordinates: Long: 4,0783; Lat: 51,37 [WGS84] ANE, Netherlands, Westerschelde, Vlissingen Stations [Marine Regions] S01 Coordinates: Long: 3,57; Lat: 51,4167 [WGS84] Belgium, Zeeschelde, Antwerp Stations [Marine Regions] S22 Coordinates: Long: 4,3917; Lat: 51,2188 [WGS84] Belgium, Zeeschelde, Doel Stations [Marine Regions] S15 Coordinates: Long: 4,2733; Lat: 51,3133 [WGS84] Temporal coverage 4 December 2002 - 6 December 2002 17 March 2003 - 21 March 2003 30 June 2003 - 4 July 2003 4 February 2004 - 5 February 2004 Taxonomic coverage Neomysis integer (Leach, 1814) [WoRMS] Parameters Growth factor Methodology Growth rate Methodology Mysid energy metabolism Methodology Mysid intermoult period Methodology Mysid testosteron metabolism Methodology Mysid vitelline concentration Methodology Growth factor: Moulting measuring protocol growth factor and the increase in length at each molting event (the growth factor, GF in % of the pre-molt length) Growth rate: Moulting measuring protocol growth rate length incresase (growth rate, µm/day) during IMPs Mysid energy metabolism: Cellular Energy Allocation The energy allocation of the estuarine mysid Neomysis integer were investigated using the cellular energy allocation assay (CEA), energy reserves (protein, lipid and sugar) and energy consumption (as derived from the cellular respiration rate) were integrated into a general indicator of physiological stress. Adult Neomysis integer specimens were sorted out on board, sex was not taken into account and no selection was made to avoid gravid females. The collected mysids were shock-frozen in liqui Mysid intermoult period: Moulting measuring protocol intermoult period time (days) between two successive molts (intermolt period; IMP) Mysid testosteron metabolism: LCMS testosteron In vivo exposure to testosterone: Juvenile Neomysis integer were taken from the different samplingsites during the campaigns of March and June and individually placed into 5 ml glass recipients containing 2 ml water (5 PSU). Two micrograms of testosterone dissolved in 10 µl methanol was added to the recipient and a 6 h exposure period was applied. Extraction and LC-MSn analysis of testosterone metabolites and endogenous steroids: N. integer were isolated from the exposure medium, shock- Mysid vitelline concentration: Enzyme-linked immunosorbent assay (ELISA) -Vitellin purification (gel filtration) -polyclonal anitbody production against vitellin -development of ELISA protocol Contributors Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Toegepaste ecologie en milieubiologie; Laboratorium voor Milieutoxicologie en aquatische ecologie; Onderzoeksgroep voor Milieutoxicologie (ECOTOX), more, data creator, data manager, data owner Belgian Science Policy (BELSPO), more, data owner Related datasets Project ENDIS-RISKS: Endocrine disruption in the Scheldt Estuary: distribution, exposure and effects, more Publication Based on this dataset Ghekiere, A. et al. (2006). Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea), in: Ghekiere, A. Studie van invertebraat-specifieke effecten van endocrien-verstorende stoffen in de estuariene aasgarnaal Neomysis integer (Leach, 1814) = Study of invertebrate-specific effects of endocrine disrupting chemicals in the estuarine mysid Neomysis integer (Leach, 1814). pp. 24-35, more Ghekiere, A. et al. (2004). Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea). Comp. Biochem. Physiol. 138(4): 427-433. https://dx.doi.org/10.1016/j.cbpb.2004.05.012, more Dataset status: Completed Data type: Data Data origin: Research Metadatarecord created: 2004-05-10 Information last updated: 2017-11-17 | ||||
