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GRL: GEANS Reference Library Citation Christodoulou M.; Beentjes K.; van der Hoorn B., Van Den Bulcke, L., Derycke S., De Backer, A.; Martinez Arbizu P.; 2021; GEANS Reference Library dx.doi.org/10.5883/DS-GEANS1 Contact: Martinez Arbizu, Pedro ; Availability: Unrestricted The data are freely available to anybody and may be used for any purpose. Usage acknowledgement may be required Description Benthic macroinvertebrates are key components in environmental impact assessments. Nevertheless, their use as bioindicators can be constrained by the time- and cost-consuming processes needed for their morphological identification. The recent advances in high-throughput sequencing, particularly DNA metabarcoding can provide an alternative to morphology-based approaches. The main limitation for DNA-based tools to be implemented in biomonitoring projects is the considerable investment needed to build high-quality and curated taxonomic reference DNA sequence libraries for species identification. To begin addressing this shortage, GEANS project aims in developing a curated DNA reference library based on mitochondrial cytochrome c oxidase subunit I (COI) for the North Sea macrobenthos that will serve as the backbone of all the molecular protocols. more For the collection of the macroinvertebrates, targeted sampling campaigntake place across the North Sea Region, while existing collections are being scanned. Macrobenthic specimens are being processed following a series of standardise work flows covering sampling, morphological identifications, molecular lab processing and data handling. All specimens are photographed and together with the accompanying sequences, collection and taxonomic data are archived in a dedicate BOLD project. Voucher specimens and DNA extracts are archived at three different institutes in Germany (Senckenberg am Meer), the Netherlands (Naturalis) and Belgium (ILVO). Once available, this DNA-library will be based on taxonomically well-curated specimens and it will support the implementation of fast, cost-efficient and reliable DNA-based identifications and subsequently environmental health assessments in the North Sea. Scope Themes: Biology, Biology > Benthos, Biology > Benthos > Macrobenthos Keywords: Marine/Coastal, Brackish water, Biodiversity, COI, Dna, DNA barcoding, Genetic, Genetic data, Marine, ANE, North Sea, Animalia Geographical coverage ANE, North Sea [Marine Regions] Temporal coverage 1 March 2019 - 28 February 2022 Quasi continuous (< 1 min.) Taxonomic coverage Animalia [WoRMS] Contributors Related datasets Parent dataset: GEANS Data: Genetic tools for Ecosystem health Assessment in the North Sea region, more Project GEANS: Genetic Tools for Ecosystem Health Assessment in the North Sea Region, more Dataset status: In Progress Data type: Data Data origin: Data collection Metadatarecord created: 2020-03-20 Information last updated: 2023-10-05 | ||||
