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gENESIS results 2022 Citation Mercurio, S.; Pennati, R.; Università degli Studi di Milano: Italy; (2022): gENESIS results 2022. https://marineinfo.org/id/dataset/8063 Contact: Availability: Unrestricted after moratorium period Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed Description COVID-19 pandemic strongly affected gENESiS, drastically reducing project length. We managed to perform some immunolocalization with rhodamine phalloidin on crinoid embryos and tried out a new in situ hybridization technique called HCR in situ with specific probes for the evolutionary conserved muscle terminal differentiation gene myosin heavy chain (mhc). Using this new approach we did obtain very nice results, with detection through the HCR in situ approach of an expression pattern for mhc than was similar than that classically obtained with the common chromogenic in situ hybridization technique. Scope Themes: Biology > Benthos Keywords: Marine/Coastal, Crinoids, Sea urchins, ANE, France, Mediterranean Sea, Echinodermata Geographical coverage ANE, France, Mediterranean Sea Taxonomic coverage Echinodermata [WoRMS] Parameter Project ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, more Dataset status: Completed Data type: Data Data origin: Research: lab experiment Metadatarecord created: 2022-05-03 Information last updated: 2022-05-03 | ||||
