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Immune receptor- and transcription factor-stimulatory activity of bacteria and endotoxin in sea spray aerosols on human cells Citable as data publication Li, Y.; Schütte, W.; Dekeukeleire, M.; Janssen, C.; Boon, N.; Asselman, J.; Lebeer, S.; Spacova, I.; De Rijcke, M.; Flanders Marine Institute (VLIZ); University of Antwerp; Ghent University: Belgium; (2024): Immune receptor- and transcription factor-stimulatory activity of bacteria and endotoxin in sea spray aerosols on human cells. Marine Data Archive. https://doi.org/10.14284/658 Contact: Li, Yunmeng Availability:  This dataset is licensed under a Creative Commons Attribution 4.0 International License.Description Total bacterial count and endotoxin concentration in sea spray aerosols, and their immunostimulatory activity on TLR4 in HEK-Blue hTLR4 cells, TLR2/6 in HEK-Blue hTLR2/6 cells, as well as NF-κB and IRF in THP1-Dual monocytes. Scope Themes: Biology Keywords: Marine/Coastal, Endotoxin, Human cells, Immunostimulatory activity, Sea spray aerosols, ANE, Belgium, Oostende Harbour, Bacteria Geographical coverage ANE, Belgium, Oostende Harbour [Marine Regions] Temporal coverage 20 March 2023 - 13 April 2023 Taxonomic coverage Bacteria [WoRMS] Parameters Endotoxin Methodology IRF activation Methodology NF-κB activation Methodology Sodium (Na) Methodology TLR2/6 activation Methodology TLR4 activation Methodology Total bacterial count Methodology Endotoxin: PyroGene Recombinant Factor C (rFC) assay IRF activation : THP1-Dual monocytes NF-κB activation : THP1-Dual monocytes Sodium (Na): Inductively Coupled Plasma Optical Emission spectroscopy TLR2/6 activation: HEK-Blue hTLR2/6 cells TLR4 activation : HEK-Blue hTLR4 cells Total bacterial count: Flow cytometry Contributors Vlaams Instituut voor de Zee (VLIZ), more, data creator University of Antwerp; Department of Bioscience Engineering; Laboratory of Applied Microbiology and Biotechnology (ENdEMIC), more, data creator Ghent University; Blue Growth Research Lab, more, data creator Ghent University; Faculty of Bioscience Engineering; Department of Biotechnology; Center for Microbial Ecology and Technology (CMET), more, data creator Publication Based on this dataset Li, Y. et al. (2024). The immunostimulatory activity of sea spray aerosols: Bacteria and endotoxins activate TLR4, TLR2/6, NF-κB and IRF in human cells. Sci. Total Environ. 927: 171969. https://dx.doi.org/10.1016/j.scitotenv.2024.171969, more Dataset status: Completed Data type: Data Data origin: Research: experiment Metadatarecord created: 2024-03-07 Information last updated: 2024-03-11 | ||||
