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Nanopore based eDNA metabarcoding of marine vertebrates in the North Sea
Citation
Doorenspleet, K.; Jansen, L.; Oosterbroek, S.; Kamermans, P.; Wurz, E.; Murk, A.; Nijland, R.; Marine Animal Ecology; Wageningen Marine Research; Wageningen University and Research Centre: The Netherlands; (2024): Nanopore based eDNA metabarcoding of marine vertebrates in the North Sea. https://marineinfo.org/id/dataset/8601

Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Description
This data is the result of DNA-based water sampling of eDNA in different locations in the North Sea. more

Molecular Data generation of marine biodiversity data plays a central role in monitoring and better understanding of North Sea marine ecosystems. By applying DNA-based techniques, this study specifically aims to present a metabarcoding pipeline for Nanopore sequencing and compare a newly designed 2kb primer with commonly used MiFish pimer. eDNA samples were taken in The ray reef aquarium in Dolfinarium , The brokum reef ground in the North sea, at sany bottom, Gmini windpark and around 3 shipwrecks in the North Sea and analysed through metabarcoding with nanopore sequencing and results were compared with morphology and commonly used MiFish primer in order to demonstrate the value of applying this technique.

Scope
Themes:
Biology > Fish, Biology > Invertebrates
Keywords:
Marine/Coastal, Elasmobranchs, Environmental DNA (eDNA), Fish, Metabarcoding, MinION, ANE, North Sea

Geographical coverage
ANE, North Sea [Marine Regions]

Temporal coverage
1 July 2019 - 31 July 2020

Parameters
Depth Methodology
pH Methodology
Salinity Methodology
Temperature Methodology
Depth: Diving computer
pH: CTD
CTD type: SBE-19 (www.seabird.com); depth profiles of temperature and salinity equipped with a OBS probe for relative turbidity measurements
Salinity: CTD
CTD type: SBE-19 (www.seabird.com); depth profiles of temperature and salinity equipped with a OBS probe for relative turbidity measurements
Temperature: CTD
CTD type: SBE-19 (www.seabird.com); depth profiles of temperature and salinity equipped with a OBS probe for relative turbidity measurements

Contributors
Wageningen University and Research Centre; Marine Animal Ecology, moredata creator
Wageningen University and Research Centre; Wageningen Marine Research (WMR), moredata creator

Dataset status: Completed
Data type: Data
Data origin: Research: field experiment
Metadatarecord created: 2024-06-17
Information last updated: 2024-06-27
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy