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Purification of maturation-inducing hormone, 17 alpha, 20ß-dihydroxy-4-pregnen-3-one, receptor solubilized from isolated cortices of goldfish (Carassius auratus) oocytes
Yoshikuni, M.; Oba, Y.; Nagahama, Y. (2000). Purification of maturation-inducing hormone, 17 alpha, 20ß-dihydroxy-4-pregnen-3-one, receptor solubilized from isolated cortices of goldfish (Carassius auratus) oocytes, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 191
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
Keyword
    Marine/Coastal
Authors  Top 
  • Yoshikuni, M.
  • Oba, Y.
  • Nagahama, Y.
Abstract
    17 alpha ,20 beta -dihydroxy-4-pregnen-3-one (17 alpha ,20 beta -DP)-binding activity was solubilized and partially purified from isolated cortices of goldfish oocytes. Several major protein bands (95, 66, and 57.5 KDa) were identified by SDS-PAGE. Interestingly, the solubilized 17 alpha ,20 beta -DP-binding activity bound to a wheat germ agglutinin (WGA)-lectin affinity column.
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