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Characterization by 16S rRNA gene analysis and in situ hybridization of bacteria living in the hindgut of a deposit-feeding echinoid (Echinodermata)
da Silva, S.G.; Gillan, D.C.; Dubilier, N.; De Ridder, C. (2006). Characterization by 16S rRNA gene analysis and in situ hybridization of bacteria living in the hindgut of a deposit-feeding echinoid (Echinodermata). J. Mar. Biol. Ass. U.K. 86(5): 1209-1213. dx.doi.org/10.1017/S0025315406014202
In: Journal of the Marine Biological Association of the United Kingdom. Cambridge University Press/Marine Biological Association of the United Kingdom: Cambridge. ISSN 0025-3154; e-ISSN 1469-7769, more
Peer reviewed article  
Available in  Authors 
Keywords
    Biodiversity
    Biology > Genetics
    Body parts > Digestive system > Digestive tract > Digestive system > Intestines > Digestive system > Hindgut
    Classification > Taxonomy > Chemotaxonomy
    Clones
    Isolating mechanisms > Genetic isolation
    Microflora
    Microorganisms > Bacteria
    Nucleic compounds > Glycosides > Nucleotides > Oligonucleotides
    Oligonucleotides
    Organisms > Microorganisms > Prokaryotes > Microorganisms > Bacteria > Filamentous bacteria
    Sediments > Chemical sediments > Nodules
    Techniques > Cytogenetic techniques > In situ hybridization > Fluorescence in situ hybridization
    Marine/Coastal
Authors  Top 
  • da Silva, S.G., more
  • Gillan, D.C., more
  • Dubilier, N.
  • De Ridder, C., more
Abstract
    The hindgut caecum of the deposit-feeding echinoid Echinocardium cordatum harbours a symbiotic bacterial microflora, organized into layered mats around detrital particles owing to the proliferation of filamentous bacteria. The bacterial community was analysed using 16S rRNA gene analysis and fluorescence in situ hybridization. The purpose was to characterize its biodiversity and to identify its predominant members. The majority of the 16S sequences belong to the delta -Proteobacteria (61.5%), the Bacteroidetes and the Firmicutes constitute the two other main bacterial groups (respectively 23.1% and 15.4%). A total of 41% of the delta -Proteobacteria clones isolated belong to a bacterium of the genus Desulfonema for which a specific oligonucleotide probe was designed, enabling identification of its distribution in the nodules.
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