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Optimized feeding strategies in the larviculture of the Asian seabass Lates calcarifer
Dhert, P.; Duray, M.; Lavens, P.; Sorgeloos, P. (1990). Optimized feeding strategies in the larviculture of the Asian seabass Lates calcarifer, in: Hirano, R. et al. (Ed.) The 2nd Asian Fisheries Forum. pp. 319-323
In: Hirano, R.; Hanyu, I. (Ed.) (1990). The 2nd Asian Fisheries Forum. Asian Fisheries Society: Manila. 991 pp., more

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Keywords
    Aquatic organisms > Food organisms
    Developmental stages > Larvae > Fish larvae
    Diets
    Artemia Leach, 1819 [WoRMS]; Brachionus Pallas, 1766 [WoRMS]; Lates calcarifer (Bloch, 1790) [WoRMS]
    ISEW, Philippines [Marine Regions]
    Marine/Coastal

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Abstract
    This paper reports on the progress made at the Tigbauan hatchery of SEAFDAC in the Philippines, with the larviculture of the Asian seabass Lates calcarifer when using w3-HUFA enriched Brachionus and Artemia .In order to optimize the transitions of the different prey types, larval mouth size was analyzed as a function of larval development: San Francisco Bay (SFB) type Artemia nauplii can be ingested from days 8 to 10 onwards, and Great Salt Lake (GSL) nauplii from days 10 to 15 (with some variations depending on growth differences between culture trials). In view of the high correlation between mouth size and total larval length, the feeding of different size classes of Artemia can be better programmed, i.e., SFB nauplii can be fed when fish larvae measure 4 mm in length, and 24 hr enriched GSL can be given to 7 mm larvae. The incorporation of the HUFA's 20:5w3 and 22:6w3 in the live prey Artemia, and possibly Brachionus, greatly improved larval ability to metamorphose, although it did not affect growth nor survival until day 21. However, when 21 day old fry were subjected to a stress test, much higher survival rates were obtained in HUFA-enriched seabass larvae, illustrating their superior physiological condition over fry cultured with non-enriched Brachionus and Artemia . These findings were used to propose an improved feeding strategy for the larviculture of Lates calcarifer.

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