Dose and time response of ruminally infused algae on rumen fermentation characteristics, biohydrogenation and Butyrivibrio group bacteria in goats
Zhu, H.; Fievez, V.; Mao, S.; He, W.; Zhu, W. (2016). Dose and time response of ruminally infused algae on rumen fermentation characteristics, biohydrogenation and Butyrivibrio group bacteria in goats. Journal of Animal Science and Biotechnology 7: 22. https://dx.doi.org/10.1186/s40104-016-0080-1 In: Journal of Animal Science and Biotechnology. BMC: London. ISSN 1674-9782; e-ISSN 2049-1891, more |   |
| Author keywords | Algae; Biohydrogenation; Goat; Hydrogenating bacteria |
| Authors | | Top | - Zhu, H.
- Fievez, V., more
- Mao, S.
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| Abstract | Background: Micro-algae could inhibit the complete rumen BH of dietary 18-carbon unsaturated fatty acid (UFAs). This study aimed to examine dose and time responses of algae supplementation on rumen fermentation, biohydrogenation and Butyrivibrio group bacteria in goats. Methods: Six goats were used in a repeated 3 x 3 Latin square design, and offered a fixed diet. Algae were infused through rumen cannule with 0 (Control), 6.1 (L-Alg), or 18.3 g (H-Alg) per day. Rumen contents were sampled on d 0, 3, 7, 14 and 20. Results: H-Alg reduced total volatile fatty acid concentration and acetate molar proportion (P < 0.05), and increased propionate molar proportion (P < 0.05), whereas L-Alg had no effect on rumen fermentation. Changes in proportions of acetate and propionate in H-Alg were obvious from d 7 onwards and reached the largest differences with the control on d 14. Algae induced a dose-dependent decrease in 18:0 and increased trans-18:1 in the ruminal content (P < 0.05). H-Alg increased the concentrations of t9, t11-18:2 and t11, c15-18:2 (P < 0.05). L-Alg only seemed to induce a transient change in 18-carbon isomers, while H-Alg induced a rapid elevation, already obvious on d 3, concentrations of these fatty acid rose in some cases again on d 20. Algae had no effect on the abundances of Butyrivibrio spp. and Butyrivibrio proteoclasticus (P > 0.10), while H-Alg reduced the total bacteria abundance (P < 0.05). However, this was induced by a significant difference between control and H-Alg on d 14 (-4.43 %). Afterwards, both treatments did not differ as increased variation in the H-Alg repetitions, with in some cases a return of the bacterial abundance to the basal level (d 0). Conclusions: Changes in rumen fermentation and 18-carbon UFAs metabolism in response to algae were related to the supplementation level, but there was no evidence of shift in ruminal biohydrogenation pathways towards t10-18:1. L-Alg mainly induced a transient effect on rumen biohydrogenation of 18-carbon UFAs, while H-Alg showed an acute inhibition and these effects were not associated with the known hydrogenating bacteria. |
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