Chemical structure and biological properties of sulfated fucan from the sequential extraction of subAntarctic Lessonia sp (Phaeophyceae)
Leal, D.; Mansilla, A.; Matsuhiro, B.; Moncada-Basualto, M.; Lapier, M.; Maya, J.D.; Olea-Azar, C.; De Borggraeve, W.M. (2018). Chemical structure and biological properties of sulfated fucan from the sequential extraction of subAntarctic Lessonia sp (Phaeophyceae). Carbohydrate Polymers 199: 304-313. https://dx.doi.org/10.1016/j.carbpol.2018.07.012 In: Carbohydrate Polymers. Elsevier SCI Ltd: Oxford. ISSN 0144-8617; e-ISSN 1879-1344, more |  |
| Keyword | | | Author keywords | Fucoidan; Sulfated fucan; Antioxidant; Antiparasitic; Biologicalactivities |
| Authors | | Top | - Leal, D.
- Mansilla, A.
- Matsuhiro, B.
- Moncada-Basualto, M.
| - Lapier, M.
- Maya, J.D.
- Olea-Azar, C.
- De Borggraeve, W.M., more
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| Abstract | This work is related to the structural characterization of the sulfated polysaccharide from Lessonia sp and the study of its antioxidant and antiparasitic properties. Sequential extraction afforded D-mannitol as the only low MW sugar alcohol. Extraction with 2% CaCl2 afforded in 3.0% yield, a sulfated fucan (SF). Its major fraction (48.5% yield), isolated by ion-exchange chromatography corresponds to a linear polymer of α-L-fucopyranosil residues linked 1→3, sulfated at the O–4 and partially at O–2 positions. By alkaline extraction, sodium alginate (10.3% yield) was obtained. The antioxidant capacity of SF by ESR showed high elimination index (IC50, mg/mL) of hydroxyl (0.27), alkoxy (10.05), and peroxyl (82.88) radicals in relation to commercial mannitol. SF showed activity against the epimastigote form of Trypanosoma cruzi parasite (250 μg/mL) and low cytotoxicity in murine cells (367 μg/mL). The elimination capacity of radicals in aqueous medium of SF would allow its potential biomedical application. |
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