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Evaluation of AFLP markers to reveal genetic diversity in Typha
Lamote, V.; De Loose, M.; Van Bockstaele, E.; Roldán-Ruiz, I. (2005). Evaluation of AFLP markers to reveal genetic diversity in Typha. Aquat. Bot. 83(4): 296-309. dx.doi.org/10.1016/j.aquabot.2005.05.013
In: Aquatic Botany. Elsevier Science: Tokyo; Oxford; New York; London; Amsterdam. ISSN 0304-3770; e-ISSN 1879-1522, more
Peer reviewed article  
Available in  Authors 
Keywords
    Aflp
    Behaviour > Social behaviour > Heat regulation > Animal behaviour > Clustering
    Cloning
    Clustering
    Genetic diversity
    Reproduction
    Typha
    Belgium, Flanders: North [Marine Regions]; Belgium, IJzer R. [Marine Regions]; Belgium, Schelde R. [Marine Regions]
    Fresh water
Author keywords
    clonal reproduction; AFLP; genetic diversity; clustering; Typha
Authors  Top 
  • Lamote, V.
  • De Loose, M.
  • Van Bockstaele, E.
  • Roldán-Ruiz, I., more
Abstract
    We investigated to what extent DNA-markers can assist species determination in the genus Typha. A set of AFLP markers was used to discriminate samples of the species Typha latifolia and Typha angustifolia collected in Flanders (North Belgium). The T. latifolia samples formed a compact cluster while the T. angustifolia samples were divided into smaller groups. It was not clear whether interspecific hybrids or higher levels of diversity present in the T. angustifolia dataset could account for this. As in previous surveys, using isozyme and VNTR markers, AFLP markers revealed an almost complete lack of genetic variation in Flemish T. latifolia. Despite the low degree of diversity, a significant level of genetic differentiation was found between the T. latifolia samples originating from different river basins. Whether this differentiation has any ecological relevance remains to be investigated. The methodology applied was not able to detect clonal reproduction in T. latifolia. Probably, the low levels of diversity present in this species can account for this, indicating that the usefulness of the methodology applied depends on the level of diversity present in the species studied.
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