IMIS

Samples were taken by 7 L Niskin bathometer at standard depths: 0, 10, 25, 50 and 100m. Samples were concentrated by reverse filtration through nucleoporous (nuclear) filters with 1 µm size pores. Fixation - 40% Formalgedid or Glutar. Cell counts were done with a light microscope (magnification 100, 200, 400 x). In addition samples were taken by 7 L Niskin bathometer from depths where irradiance level = 100, 46, 25, 10 and 1% and were processed without concentration in drop 0.1-0.5 ml (1-3 replications). Individual biovolumes and biomass (wet weight) were calculated via geometric approximations. At IBSS, the data were digitized from paper processing books, quality-controlled and transformed to comply with the Darwin Core/OBIS Schema. Funds for data operations were kindly provided by the Census of Marine Life International Cosmos Prize Fund, through a grant to Rutgers University.