IMIS

A new integrated search interface will become available in the next phase of marineinfo.org.
For the time being, please use IMIS to search available data

 

[ report an error in this record ]basket (1): add | show Print this page
one publication added to basket [310160]
Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates
Peeters, E.; Nelis, H.J.; Coenye, T. (2008). Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J. microbiol. methods 72(2): 157-165. https://dx.doi.org/10.1016/j.mimet.2007.11.010
In: Journal of Microbiological Methods. Elsevier: Amsterdam. ISSN 0167-7012; e-ISSN 1872-8359, more
Peer reviewed article  
Available in  Authors 
Author keywords
    Biofilm quantification; Microtiter plate; Crystal violet; Resazurine; FDA; XTT
Authors  Top 
  • Peeters, E.
  • Nelis, H.J.
  • Coenye, T., more
Abstract
    In the present study six assays for the quantification of biofilms formed in 96-well microtiter plates were optimised and evaluated: the crystal violet (CV) assay, the Syto9 assay, the fluorescein diacetate (FDA) assay, the resazurin assay, the XTT assay and the dimethyl methylene blue (DMMB) assay. Pseudomonas aeruginosa, Burkholderia cenocepacia, Staphylococcus aureus, Propionibacterium acnes and Candida albicans were used as test organisms. In general, these assays showed a broad applicability and a high repeatability for most isolates. In addition, the estimated numbers of CFUs present in the biofilms show limited variations between the different assays. Nevertheless, our data show that some assays are less suitable for the quantification of biofilms of particular isolates (e.g. the CV assay for P. aeruginosa).
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors