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Supplementary information accompanying the paper "Nematodes facilitate bacterial-mediated biogeochemical processes in marine sediments: a meta-transcriptomics approach" Citable as data publication Guden, R.M.E.; Haegeman, A.; Ruttink, T.; Moens, T.; Derycke, S.; Marine Biology Research Group - Ugent; Institute for Agricultural and Fisheries Research (ILVO): Belgium; (2022): Supplementary information accompanying the paper "Nematodes facilitate bacterial-mediated biogeochemical processes in marine sediments: a meta-transcriptomics approach". Marine Data Archive. https://doi.org/10.14284/575 Contact: Guden, Rodgee Mae Availability:  This dataset is licensed under a Creative Commons Attribution 4.0 International License.Description This study used metatranscriptomics to investigate taxonomic and functional shifts in marine sediment bacterial communities in response to the addition of a nematode community.
The scripts and data in this repository are accompanying the paper "Nematodes facilitate bacterial-mediated biogeochemical processes in marine sediments: a meta-transcriptomics approach" by Rodgee Mae Guden, Annelies Haegeman, Tom Ruttink, Tom Moens and Sofie Derycke (Ghent University / ILVO, Belgium). The scripts are also available on Gitlab, with an overview of the bioinformatics pipeline. more Our study investigated the taxonomic and functional shifts in benthic bacterial communities in response to the addition of a nematode community using a metatranscriptomic approach. We created 10 microcosms comprising sediment and natural bacterial populations, and extracted RNA at the start of the experiment. Natural nematode communities from an intertidal mudflat were then added to five of the microcosms. After seven days of incubation, RNA was extracted from the microcosms to perform metatranscriptomics analysis to investigate the taxonomic and functional composition of the samples. Scope Themes: Biology > Benthos > Meiobenthos, Biology > Ecology - biodiversity Keywords: Marine/Coastal, Bacteria, Carbon and nitrogen cycling, Cluster of Orthologous genes (COGs), Ecosystem functioning, Functional annotation, Marine biogeochemistry, Metatranscriptomics, Nematode composition, Sediment microbiome, Taxonomic composition of bacterial community, ANE, Netherlands, Westerschelde, PaulinaPolder Geographical coverage ANE, Netherlands, Westerschelde, PaulinaPolder Contributors Dataset status: Completed Data type: Data Data origin: Research Metadatarecord created: 2022-09-13 Information last updated: 2022-09-15 | ||||
