Norchlorotestosterone acetate: an alternative metabolism study and GC-MS2 analysis in kidney fat, urine, and faeces
Van Hoof, N.; De Wash, K.; Poelmans, S.; Bruneel, D.; Spruyt, S.; Noppe, H.; Janssen, C.R.; Courtheyn, D.; De Brabander, H.F. (2004). Norchlorotestosterone acetate: an alternative metabolism study and GC-MS2 analysis in kidney fat, urine, and faeces. Chromatographia (Wiesb.) 59: S85-S93. https://dx.doi.org/10.1365/s10337-003-0178-4 In: Chromatographia. Vieweg Verlag: Heidelberg; Berlin. ISSN 0009-5893; e-ISSN 1612-1112, more |  |
| Keywords | Analysis > Chemical analysis
Analytical techniques > Chromatographic techniques > Gas chromatography
Atmospheric pressure
Chlorine organic compounds
Electron impact
Feces
HPLC
Kidneys
Mass spectrometry
Mass spectrometry
Metabolism
Metabolites
Qualitative analysis
Steroids
Techniques > Methodology > Analytical methods > Spectroscopy > Spectrometry > Mass spectrometry
Urine
Arthropoda [WoRMS]; Crustacea [WoRMS]; Invertebrata; Mysida [WoRMS]; Neomysis integer (Leach, 1814) [WoRMS]
Fresh water |
| Authors | | Top | - Van Hoof, N.
- De Wash, K.
- Poelmans, S., more
| - Bruneel, D.
- Spruyt, S.
- Noppe, H., more
| - Janssen, C.R., more
- Courtheyn, D.
- De Brabander, H.F., more
|
| Abstract | Norchlorotestosterone acetate (NCITA) is an anabolic steroid which resembles chlorotestosterone acetate. It cannot yet be detected by routine methods used for anabolic steroids, because there is no knowledge of its metabolic pathway. The invertebrate Neomysis integer has been used as an alternative model to study the metabolism of NCITA. The experimental results indicated the presence of 4-norchloroandrost-4-ene-17-ol-3-one (NCIT) and 4-norchloroand-rost-4-ene-3,17-dione (NorCIAD) as possible metabolites of NCITA. Subsequently NCITA and the synthesised metabolites NCIT and NorCIAD were incorporated into the routine multi-residue method for detection of anabolic steroids in kidney fat, urine, and faeces. |
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