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Identification of cod fillets by HPLC: a screening method
Vyncke, W. (1992). Identification of cod fillets by HPLC: a screening method. Mededelingen van het Rijksstation voor Zeevisserij (CLO Gent), 231. Rijkscentrum voor Landbouwkundig Onderzoek: Gent. 12 pp.
Part of: Mededelingen van het Rijksstation voor Zeevisserij (CLO Gent). Rijksstation voor Zeevisserij: Oostende. ISSN 0303-9072, more
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Keywords
    HPLC
    Identification
    Gadiformes [WoRMS]; Gadus morhua Linnaeus, 1758 [WoRMS]
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Abstract
    Water soluble sarcoplasmic proteins of nine Gadoid species were chromatographed using a C4 wide pore reversed phase column and gradient elution with trifluoracetic acid, water and acetonitrile. In most cases, nine peaks could be identified in cod (Gadus morhua), with four major peaks always present. Direct visual comparison of unknown chromatograms with a cod chromatogram kept in memory by the data processor allowed the elimination of most non-cod Gadoids. Appearance of peaks not found in cod confirmed this elimination. In case of doubt, however, confirmation by electrophoretic technics is recommended. HPLC appeared to be a quick screening technique for identifying cod by eliminating non-cod species.
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