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Thermoanaerobaculum aquaticum gen. nov., sp. nov., the first cultivated member of Acidobacteria subdivision 23, isolated from a hot spring
Losey, N.A.; Stevenson, B.S.; Busse, H.-J.; Sinninghe Damsté, J.S.; Rijpstra, W.I.C.; Rudd, S.; Lawson, P.A. (2013). Thermoanaerobaculum aquaticum gen. nov., sp. nov., the first cultivated member of Acidobacteria subdivision 23, isolated from a hot spring. Int. J. Syst. Evol. Microbiol. 63: 4149-4157. http://dx.doi.org/10.1099/ijs.0.051425-0
In: International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology: Reading. ISSN 1466-5026; e-ISSN 1466-5034, more
Peer reviewed article  
Authors  Top 
  • Losey, N.A.
  • Stevenson, B.S.
  • Busse, H.-J.
  • Sinninghe Damsté, J.S., more
  • Rijpstra, W.I.C., more
  • Rudd, S.
  • Lawson, P.A.
Abstract
    A novel bacterium was isolated from a freshwater hot spring, the Hale House Spring, located at Hot Springs National Park, Hot Springs, AR, USA. Cells of strain MP-01T stained Gram-negative, were rod-shaped, non-motile, strictly anaerobic and chemo-organotrophic and did not form spores. Growth occurred at 50–65 °C, with an optimum at 60 °C, at pH 6.0–8.0, with an optimum at pH 6.5–7.0, and at NaCl concentrations up to 0.5?% (w/v), with optimum growth in the absence of NaCl. Strain MP-01T was capable of fermentative growth on pyruvate or proteinaceous substrates as well as reducing Fe(III) and Mn(IV). Major fatty acids were iso-C15?:?0, iso-C16?:?0, anteiso-C17?:?0 and iso-C17?:?0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and the major isoprenoid quinone was MK-10. In the polyamine pattern, sym-homospermidine was the predominant compound. The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that strain MP-01T represents the first reported cultivated member of subdivision 23 of the Acidobacteria. It is proposed that strain MP-01T represents a novel genus and species, for which the name Thermoanaerobaculum aquaticum gen. nov., sp. nov. is proposed. The type strain of Thermoanaerobaculum aquaticum is MP-01T (?=?DSM 24856T?=?JCM 18256T).
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