Colonized beads as inoculum for marine biodegradability assessment: application to Linear Alkylbenzene Sulfonate
Mauffret, A.; Rottiers, A.; Federle, T.; Gillan, D.C.; Hampel, M.; Blasco, J.; Temara, A. (2009). Colonized beads as inoculum for marine biodegradability assessment: application to Linear Alkylbenzene Sulfonate. Environ. Int. 35(6): 885-892. http://dx.doi.org/10.1016/j.envint.2009.03.007 In: Environment International. Pergamon: New York. ISSN 0160-4120; e-ISSN 1873-6750, more |  |
| Keyword | | | Author keywords | Marine; Biodegradation; LAS; Colonized beads; Biofilm |
| Authors | | Top | - Mauffret, A., more
- Rottiers, A.
- Federle, T.
- Gillan, D.C., more
| - Hampel, M.
- Blasco, J.
- Temara, A., more
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| Abstract | An innovative biodegradation test system was developed in order to fill the current gap for cost effective and environmentally relevant tools to assess marine biodegradability. Glass beads were colonized by a biofilm in an open flow-through system of seawater with continuous pre-exposure to Linear Alkylbenzene Sulfonate (LAS) (20 µg/L). Thereafter, such colonized beads were added as inoculum in different test systems. [14C]-LAS (5–100 µg/L) was added and primary and ultimate biodegradation were assessed. The bacterial density collected on the beads (109 bact./mL beads) was ca. 3 orders of magnitude higher than the typical seawater content. The LAS mineralization lag phase duration decreased from 55 to < 1 days and the mineralization extent increased from 53 to 90% as the colonized beads volume increased from 10 to 275 mL. This is the first demonstration of marine bacteria's ability to mineralize LAS. On the opposite, less than 13% LAS was mineralized in seawater only. The colonized beads possibly enhanced the probability to encounter the full degraders' consortium in a low volume of seawater (100 mL). |
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