In vitro pharmacological and toxicological effects of Norterpene Peroxides isolated from the Red Sea sponge Diacarnus erythraeanus on normal and cancer cells
Lefranc, F.; Nuzzo, G; Hamdy, A; Fakhr, I; Banuls, L.M.Y.; Van Goietsenoven, G.; Villani, G; Mathieu, V.; van Soest, R; Kiss, R.; Ciavatta, L (2013). In vitro pharmacological and toxicological effects of Norterpene Peroxides isolated from the Red Sea sponge Diacarnus erythraeanus on normal and cancer cells. J. Nat. Prod. 76(9): 1541-1547. http://dx.doi.org/10.1021/np400107t In: Journal of Natural Products. American Chemical Society/American Society of Pharmacognosy: Washington DC. ISSN 0163-3864; e-ISSN 1520-6025, more |  |
| Keywords | Diacarnus erythraeanus Kelly-Borges & Vacelet, 1995 [WoRMS]
Marine/Coastal |
| Authors | | Top | - Lefranc, F.
- Nuzzo, G
- Hamdy, A
- Fakhr, I
| - Banuls, L.M.Y.
- Van Goietsenoven, G.
- Villani, G
- Mathieu, V., more
| - van Soest, R
- Kiss, R., more
- Ciavatta, L
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| Abstract | Eight cyclic peroxide norterpenoids, compounds 1-8, have been isolated and characterized from the Red Sea sponge Diacarnus erythraeanus, including two new norsesterterpene derivatives (3, 4). Among these metabolites, (-)-muqubilin A (5) (nine cell lines analyzed) and the new compounds 3 and 4 (seven cell lines analyzed) displayed mean IC50 growth inhibitory concentrations in vitro of <10 mu M, while the remaining compounds (1, 6-8) were inactive in these cancer cell lines. Compound 5 displayed no selectivity between normal and cancer cells in terms of in vitro growth inhibition. Quantitative video microscopy analysis carried out on (-)-muqubilin A-treated cells validated the data obtained by means of the MTT colorimetric assay, while flow cytometry analysis revealed ROS production but no induction of apoptosis in cancer cells. |
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