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Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life
Rimet, F.; Aylagas, E.; Borja, A.; Bouchez, A.; Canino, A.; Chauvin, C.; Chonova, T.; Ciampor Jr, F.; Costa, F.O.; Ferrari, B.J.D.; Gastineau, R.; Goulon, C.; Gugger, M.; Holzmann, M.; Jahn, R.; Kahlert, M.; Kusber, W.-H.; Laplace-Treyture, C.; Leese, F.; Leliaert, F.; Mann, D.G.; Marchand, F.; Méléder, V.; Pawlowski, J.; Rasconi, S.; Rivera, S.; Rougerie, R.; Schweizer, M.; Trobajo, R.; Vasselon, V.; Vivien, R.; Weigand, A.; Witkowski, A.; Zimmermann, J.; Ekrem, T. (2021). Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life. Metabarcoding and Metagenomics 5: e58056. https://dx.doi.org/10.3897/mbmg.5.58056
In: Metabarcoding and Metagenomics. Pensoft Publishers: Sofia. e-ISSN 2534-9708, more
Peer reviewed article  
Available in  Authors 
Author keywords
    aquatic organisms, barcode, DNA, metadata, quality, reference library, traceability
Authors  Top 
  • Rimet, F., more
  • Aylagas, E.
  • Borja, A., more
  • Bouchez, A.
  • Canino, A.
  • Chauvin, C.
  • Chonova, T.
  • Ciampor Jr, F.
  • Costa, F.O.
  • Ferrari, B.J.D.
  • Gastineau, R.
  • Goulon, C.
  • Gugger, M.
  • Holzmann, M.
  • Jahn, R.
  • Kahlert, M.
  • Kusber, W.-H.
  • Laplace-Treyture, C.
  • Leese, F.
  • Leliaert, F., more
  • Mann, D.G., more
  • Marchand, F.
  • Méléder, V.
  • Pawlowski, J.
  • Rasconi, S.
  • Rivera, S.
  • Rougerie, R.
  • Schweizer, M.
  • Trobajo, R.
  • Vasselon, V.
  • Vivien, R.
  • Weigand, A.
  • Witkowski, A., more
  • Zimmermann, J.
  • Ekrem, T.
Abstract
    DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.
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