Induction of transgenerational innate immune memory against Vibrio infections in a brine shrimp (Artemia franciscana) model
Roy, S.; Baruah, K.; Bossier, P.; Vanrompay, D.; Norouzitallab, P. (2022). Induction of transgenerational innate immune memory against Vibrio infections in a brine shrimp (Artemia franciscana) model. Aquaculture 557: 738309. https://dx.doi.org/10.1016/j.aquaculture.2022.738309 In: Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo. ISSN 0044-8486; e-ISSN 1873-5622, more |  |
| Keywords | Artemia franciscana Kellog, 1906 [WoRMS]; Vibrio Pacini, 1854 [WoRMS]
| | Author keywords | Transgenerational; Innate immune memory; Trained immunity; Brine shrimp; Epigenetics; vibrio; Acute hepatopancreas necrosis disease |
| Abstract | The occurrence of transgenerational innate immune memory in invertebrates possibly offers an opportunity for the creation of offspring with increased resistance against infectious diseases. Shrimps, being invertebrates rely on their innate immune system as the main defence mechanism to combat invading pathogens. Thus, creating transgenerational innate immune memory could be used for disease prevention in shrimp farming. However, the mechanisms behind the induction of transgenerational innate immune memory and the creation of a broad spectrum protective immune memory are still not fully understood. Here, the creation of transgenerational innate immune memory and the mechanisms involved were studied using the brine shrimp (Artemia franciscana) as a model for shrimps. Hereto, a parental generation was primed by exposure to a live or dead Vibrio para-haemolyticus PV1 while another parental generation was primed with live or dead Vibrio campbellii LMG21363. Both Vibrio strains are known to cause diseases in farmed shrimps, resulting in significant economic losses. Axenic larvae of three subsequent generations were exposed either to the homologous or heterologous (viable) Vibrio species and their survival was recorded. In general, progenies from primed F0 parents developed innate immune memory as compared to offspring of non-primed control F0 parents as they were significantly protected against a subsequent Vibrio infection. Protection in the infected offspring was more pronounced when they were infected with the homologous Vibrio strain. Disease resistance was associated with significantly increased transcription of especially peroxinectin (pxn), prophenoloxidase (proPO), lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP), high mobility group box 1 (HMGB1) and heat shock protein 90 (hsp90) genes and significant changes in histone H3 and H4 acetylation and m6A RNA methylation. Overall, results might lead to procedures at shrimp brood stock level for parental conditioning and creation of transgenerational innate immune memory in offspring. |
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