The effects of Pandanus tectorius leaf extract on the resistance of white-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus
Anirudhan, A.; Iryani, M.T.M.; Andriani, Y.; Sorgeloos, P.; Tan, M.P.; Wong, L.L.; Mok, W.J.; Ming, W.; Yantao, L.; Lau, C.C.; Sung, Y.Y. (2023). The effects of Pandanus tectorius leaf extract on the resistance of white-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus. Fish and Shellfish Immunology Reports 4: 100101. https://dx.doi.org/10.1016/j.fsirep.2023.100101 In: Fish and Shellfish Immunology Reports. Elsevier: United Kingdom. e-ISSN 2667-0119, more |   |
| Keywords | Pandanus tectorius; Penaeus vannamei Boone, 1931 [WoRMS]; Vibrio Pacini, 1854 [WoRMS]; Vibrio parahaemolyticus (Fujino, Okuno, Nakada, Aoyama, Fukai, Mukai & Ueho, 1951) Sakazaki, Iwanami & Fukumi, 1963 [WoRMS]
| | Author keywords | Penaeus vannamei; Pandanus tectorius; Vibrio; Immunity; Hsp70 |
| Authors | | Top | - Anirudhan, A.
- Iryani, M.T.M.
- Andriani, Y.
- Sorgeloos, P., more
| - Tan, M.P.
- Wong, L.L.
- Mok, W.J.
- Ming, W.
| - Yantao, L.
- Lau, C.C.
- Sung, Y.Y.
|
| Abstract | Pandanus tectorius leaf extract effect on the White-leg shrimp Penaeus vannamei tolerance against Vibrio parahaemolyticus were investigated in this study. Thirty shrimp post-larvae measured at approximately 1 cm were exposed for 24 h to 0.5, 1, 2, 3, 4, 5 and 6 g/L leaf extract and subsequently observed for survival and immune-related genes expression (Hsp70, ProPO, peroxinectin, penaeidin, crustin and transglutaminase), followed by determination of their tolerance and histological tissue profiles upon Vibrio challenge. Survival of shrimps treated with 6 g/L of leaf extract improved by up to 95% to controls. Hsp70, crustin, and prophenoloxidase mRNA levels were observed to be 8.5, 10.4, and 1.5-fold higher, respectively. Histopathological analysis of the hepatopancreas and the muscle tissues revealed major tissue degeneration in Vibrio-challenged shrimps but not in shrimps primed with P. tectorius leaf extract. Of all the dose examined, the best pathogen resistance results were obtained with a 24 h incubation of shrimp in 6 g/L P. tectorius methanolic leaf extract. The tolerance towards V. parahaemolyticus might be associated with the increased regulation of Hsp70, prophenoloxidase and crustin upon exposure to the extract, all immune-related proteins essential for pathogen elimination in Penaeid shrimp. The present study primarily demonstrated that P. tectorius leaf extract is a viable alternative for enhancing P. vannamei post-larvae resistance against V. parahaemolyticus, a major bacterial pathogen in aquaculture. |
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