Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina)
de Swart, R.L.; Kluten, R.M.G.; Huizing, C.J.; Vedder, L.J.; Reijnders, P.J.H.; Visser, I.K.G.; Uytdehaag, F.G.C.M.; Osterhaus, A.D.M.E. (1993). Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina). Vet. Immunol. Immunopathol. 37(3-4): 217-230. https://dx.doi.org/10.1016/0165-2427(93)90195-A In: Veterinary Immunology and Immunopathology. Elsevier: Tokyo; Oxford; Lausanne; New York; Shannon; Amsterdam. ISSN 0165-2427; e-ISSN 1873-2534, more Related to:de Swart, R.L.; Kluten, R.M.G.; Huizing, C.J.; Vedder, L.J.; Reijnders, P.J.H.; Visser, I.K.G.; Uytdehaag, F.G.C.M.; Osterhaus, A.D.M.E. (1993). Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal ( Phoca vitulina), in: de Swart, R.L. (1995). Impaired immunity in seals exposed to bioaccumulated environmental contaminants = Immuunsuppressie in zeehonden blootgesteld aan in de voedselketen geaccumuleerde milieuvervuilende stoffen. pp. 23-34, more |  |
| Keywords | Analysis > Response analysis
Antigens
Aquatic organisms > Marine organisms > Aquatic mammals > Marine mammals
Cells > Blood cells
Cells > Blood cells > Lymphocytes
Immunology
Induction
Induction
Induction
Mitogens
Phoca vitulina Linnaeus, 1758 [WoRMS]
Marine/Coastal |
| Authors | | Top | - de Swart, R.L.
- Kluten, R.M.G.
- Huizing, C.J.
- Vedder, L.J.
| - Reijnders, P.J.H., more
- Visser, I.K.G.
- Uytdehaag, F.G.C.M.
- Osterhaus, A.D.M.E.
| |
| Abstract | In vitro assays were developed for studies concerning the functioning of the immune system of the harbour seal (Phoca vitulina). Proliferative responses of peripheral blood mononuclear cells (PBMC) were measured after stimulation with different concentrations of the mitogens concanavalin A (Con A), pokeweed mitogen (PWM), phytohaemagglutinin (PHA) or lipopolysaccharide from Salmonella typhimurium (LPS). Con A and PWM induced strong proliferative responses, which PHA and LPS induced comparatively low proliferative responses. Antigen-specific immune responses were measured after immunization of seals with an inactivated rabies vaccine and/or with tetanus toxoid. Antigen-specific proliferation of PBMC and the presence of antigen-specific antibody forming cells were demonstrated for both antigen s in the PBMC of immunized animals. The responses measured in vitro correlated well with the development of specific serum antibody titers to these antigens. |
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