Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically driven continuous fluid samplers
Robidart, J.; Callister, S.J.; Song, P.; Nicora, C.D.; Wheat, C.G.; Girguis, P.R. (2013). Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically driven continuous fluid samplers. Environ. Sci. Technol. 47(9): 4399-4407. https://dx.doi.org/10.1021/es3037302
In: Environmental Science and Technology. American Chemical Society: Easton. ISSN 0013-936X; e-ISSN 1520-5851, more
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| Authors | | Top |
- Robidart, J.
- Callister, S.J.
- Song, P.
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- Nicora, C.D.
- Wheat, C.G.
- Girguis, P.R.
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| Abstract |
Microbes play a key role in mediating aquatic biogeochemical cycles. However, our understanding of the relationships between microbial phylogenetic/physiological diversity and habitat physicochemical characteristics is restrained by our limited capacity to concurrently collect microbial and geochemical samples at appropriate spatial and temporal scales. Accordingly, we have developed a low-cost, continuous fluid sampling system (the Biological OsmoSampling System, or BOSS) to address this limitation. The BOSS does not use electricity, can be deployed in harsh/remote environments, and collects/preserves samples with daily resolution for >1 year. Here, we present data on the efficacy of DNA and protein preservation during a 1.5 year laboratory study as well as the results of two field deployments at deep-sea hydrothermal vents, wherein we examined changes in microbial diversity, protein expression, and geochemistry over time. Our data reveal marked changes in microbial composition co-occurring with changes in hydrothermal fluid composition as well as the temporal dynamics of an enigmatic sulfide-oxidizing symbiont in its free-living state. We also present the first data on in situ protein preservation and expression dynamics highlighting the BOSS’s potential utility in meta-proteomic studies. These data illustrate the value of using BOSS to study relationships among microbial and geochemical phenomena and environmental conditions. |
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